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. Author manuscript; available in PMC: 2019 Aug 27.
Published in final edited form as: Cell Rep. 2019 Aug 13;28(7):1845–1859.e5. doi: 10.1016/j.celrep.2019.07.031

Figure 7. Drp1−/− Tumor Cells Exhibit Impaired Fatty Acid Trafficking.

Figure 7.

(A) Schematic of Red C12 cellular processing.

(B) Representative confocal fluorescence images of Red C12 pulse-chase over 25 h in indicated cell lines. Red C12 (fatty acids, magenta), BODIPY 493/503 (lipid droplets, green), and DAPI (nuclei, blue). Scale bars, 20 μm.

(C) Quantification using Pearson’s correlation coefficient from (B) and Figure S5. (n = 3 [128 and 109–3 lines]; n = 2 [remaining cell lines], ≥50 cells analyzed per time point per cell line).

(D) Cell expansion of indicated cell lines under specified nutrient conditions following 24 h in culture (representative result, n = 3).