. Author manuscript; available in PMC: 2019 Aug 27.

Published in final edited form as: Cell Rep. 2019 Aug 13;28(7):1845–1859.e5. doi: 10.1016/j.celrep.2019.07.031

KEY RESOURCES TABLE.

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Drp1	Abcam	Ab184247
p44/42 MAPK (Erk 1/2)	Cell Signaling Technology	CST-9102; RRID: AB_330744
phospho-p44/42 MAPK (Erk 1/2) T202/Y204	Cell Signaling Technology	CST-9101; RRID: AB_331646
HK2 (C64G5)	Cell Signaling Technology	CST-2867; RRID: AB_2232946
Hif1α	Cell Signaling Technology	CST-3716; RRID: AB_2116962
β-Tubulin (9F3)	Cell Signaling Technology	CST-2128; RRID: AB_823664
β-Actin (D6AB)	Cell Signaling Technology	CST-8457; RRID: AB_10950489
GAPDH (D16H11)	Cell Signaling Technology	CST-5174; RRID: AB_10622025
anti-Mitochondria antibody (clone 113–1, Cy3 conjugate)	EMD Millipore	MAB1273C3; RRID: AB_2631100
Biological Samples
Patient-derived xenografts (PDX)	Walters et al., 2013b	N/A
Chemicals
Atglistatin	Sigma	SML1075
Glucose, D-[3-³H]	Perkin-Elmer	NET331C001MC
Glucose, D-[¹⁴C (U)]	Perkin-Elmer	NEC042X250UC
oligomycin	Millipore	495455
2-deoxyglucose	Chem-Impex	21916
BAM15	Cayman	17811
Rotenone	Sigma	R8875
Antimycin A	Sigma	A8674
Phusion HF DNA Polymerase	New England Biolabs	M0530
EnVision FLEX Target Retrieval Solution	Dako, Glostrup, Denmark	GV80411–2 (Agilent)
3,3′-diaminobenzidine tetrahydrochloride (DAB+) chromogen	Dako, Glostrup, Denmark	GV82511–2 (Agilent)
Critical Commercial Assays
CellTiter-Glo Luminescent Cell Viability assay	Promega	G7572
CyQUANT Cell Proliferation Assay	ThermoFisher	C7026
Mitochondrial Stress Test	Seahorse Biosciences	103015–100
Glycolysis Stress Test	Seahorse Biosciences	103020–100
Lactate-Glo Luminescent assay	Promega	J5021
Experimental Models: Cell Lines
PDX 188t	Walters et al., 2013b	N/A
PDX 395t	Walters et al., 2013b	N/A
L3.6pL	Bruns et al., 1999	Gift from Dr. Kim Kelly
CFPac	Moore et al., 2001	Gift from Dr. Kim Kelly
109, 123, 128, 132, 143, 145, 253, 410, 650 (+ clones)	This Paper	N/A
P.1, KP.1, KPD.1, P.2, KP.2, KPD.2	This Paper	N/A
Experimental Models: Organisms/Strains
LSL-KRas^G12D/+; TP53^flox/flox	Jackson et al., 2005	Gift of Kwon Park
Pdx1-Cre-ER^TM	Gidekel Friedlander et al., 2009	Jackson Laboratory
Drp1^flox/flox	Wakabayashi et al., 2009	N/A
Oligonucleotides
HK2 Forward: 5′-TGATCGCCTGCTTATTCACGG-3′	Shi et al., 2011	N/A
HK2 Reverse: 5′-AACCGCCTAGAAATCTCCAGA-3′	Shi et al., 2011	N/A
ActB Forward: 5′-TGACGTTGACATCCGTAAAG-3′	Yan et al., 2016	N/A
ActB Reverse: 5′-GAGGAGCAATGATCTTGATCT-3′	Yan et al., 2016	N/A
Ubc Forward: 5′-GCCCAGTGTTACCACCAAGA-3′	Yan et al., 2016	N/A
Ubc Reverse: 5′-CCCATCACACCCAAGAACA-3′	Yan et al., 2016	N/A
Recombinant DNA
pLenti CMV TetR Blast	Campeau et al., 2009	Addgene #17492
pSuperior-Retro-Puro-scramble	Kashatus et al., 2015	N/A
pSuperior-Retro-Puro-shDrp1	Kashatus et al., 2015	Addgene #99385
Software and Algorithms
ImageScope Annotations tool	Leica Biosystems	https://www.leicabiosystems.com/digital-pathology/manage/aperio-imagescope/