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. 2019 Sep;25(9):1765–1768. doi: 10.3201/eid2509.190380

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Phylogenetic analysis of Talaromyces species based on concatenated nucleotide alignments of internal transcribed spacer, partial β-tubulin gene, and partial RNA polymerase II largest subunit gene regions, showing the relationship among clinical isolates from patients in California, USA (black squares), and reference Talaromyces species. The tree was constructed by the neighbor-joining method with 1,000 bootstrap replicates by using MEGA software (https://www.megasoftware.net). Bootstrap support values >70% are presented at the nodes. The tree was rooted with Trichocoma paradoxa CBS 788.83. GenBank accession numbers for newly generated sequences are MK601832–41 for the internal transcribed spacer, MK626499–508 for the β-tubulin gene, and MK626509–518 for the RNA polymerase II largest subunit gene. CBS, Westerdijk Fungal Biodiversity Institute; MDL, Microbial Diseases Laboratory, California Department of Public Health. Scale bar indicates estimated phylogenetic divergence.