Figure 6.

Phylogenetic analysis of E93 orthologues of Palaeoptera, Polyneoptera, Paraneoptera and Endopterygota species, as well as Thermobia domestica, as a representative of the ametabolan Zygentoma, and Catajapyx aquilonaris (Hexapoda, Diplura) as an hexapodan external group. Alignments were carried out with ClustalX [49] and phylogenetic reconstruction with RAxML [50], based on the maximum-likelihood principle, a JTT matrix, a gamma model of heterogeneity rate, and using empirical base frequencies and estimating proportions. The data were bootstrapped for 100 replicates. Bootstrap values are indicated in the subclass, superorder and order nodes. Scale bar indicates the number of substitutions per site. Complete species names and accession data of the respective sequences are detailed in the electronic supplementary material, table S1.