Table 2.

Outline of study design and specimen collections. For the vaginal PK study, female pigtailed macaques (N = 6) were treated with 0.5 or 1.5 ml IQB4012 that was applied in the posterior vagina, proximal to the cervix. For the rectal PK study, 1 or 4 ml IQB4012 was applied in the rectum of N = 6 female pigtailed macaques. Specimens indicated were collected immediately before (0 h) and at 2, 4, and 24 h after each weekly gel application

Sample	0 h*	2 h	4 h	24 h
Blooda (vaginal and rectal PK)	X	X	X	X
Vaginal multiswabb (vaginal PK)	X	X	X	X
Rectal spearsc (vaginal and rectal PK)	X	X	X	X
Vaginal pinch biopsiesd (vaginal PK)	-	-	X	X
Rectal pinch biopsiese (vaginal and rectal PK)	-	-	X	X
Vaginal pHf (vaginal PK)	X	X	X	X

^a2-ml CPT tubes

^bVaginal multiswab device loaded with eight Weck-Cel sponges; four proximal and four distal

^cTwo rectal wicks/animal/time point

^dVAGINAL: three pinch biopsies/animal/time point (one prox, one med, one dist)

^eRECTAL: two pinch biopsies/animal/time point

^fVaginal pH: pH indicator strips