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E2F1 upregulates IRF5 expression. (A–B) Quantification of IRF5 mRNA level after pE2F1-mediated overexpression of E2F1 in HCC827 cells for 48h. (C–D) Quantification of IRF5 mRNA level after E2F1 siRNA-mediated knockdown in HCC827cells for 48h. (E–F) IFR5 and E2F1 protein levels were detected by Western blot analysis in HCC827 cells after transfected with different doses E2F1 siRNA orE2F1 overexpression plasmid for 48 h. *p<0.05, **p<0.01, ***p<0.001.
