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. 2019 Aug 23;12:6907–6915. doi: 10.2147/OTT.S215701

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© 2019 Feng et al.

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E2F1 binds to the minimal promoter of IRF5 in vitro. (A) CHIP for detecting binding of E2F1 to the IRF5 promoter in vitro. The protein precipitated by the anti-E2F1 antibody binds in vitro to the amplified sequence of the IRF5 promoter, whereas the anti-IgG antibody cannot precipitate the bound protein in the sequence. (B) The immunoprecipitated chromatin fragments were analyzed by quantitative PCR using primer pairs spanning the putative E2F1 binding site.