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. Author manuscript; available in PMC: 2019 Aug 27.
Published in final edited form as: Cancer Res. 2017 Jan 20;77(7):1649–1661. doi: 10.1158/0008-5472.CAN-16-0919

Figure 2.

Figure 2

SDHD expression difference in SDHD copy-neutral melanomas harboring promoter mutations compared to wild-type samples. A, SDHD mRNA expression is significantly decreased in TCGA SKCM samples harboring the C523T and C541T mutations relative to wild-type samples. B, SDHD promoter activity is significantly decreased by SDHD hotspot mutation (C523T and C524T). A 163-bp fragment from the wild-type SDHD promoter sequence surrounding hotspot mutations significantly enhance luciferase reporter expression relative to vector control, whereas the same fragment containing hotspot mutations decrease promoter activity relative to the wild-type sequence. Fold change over minimal promoter control (vector only) is plotted as relative luciferase activity. The experiment was performed four times with triplicates for each. Stars denote significant differences in luciferase activity by two-tailed student’s t-test (*: P-value <0.05; **: P-value <0.01; ***: P-value <0.001).