Figure 3.

Predicting SDHD promoter mutation effects on transcription factor binding sites. A–B, data are shown for (A) the C523T mutation and (B) the C524T mutation. Genomic sequence and coordinates are at the bottom of the display; the positions of the matches represented (light blue boxes). The position of the mutation within the motif is indicated by a red-bounding box, with the alternate allele below in red font as on the motif logo position bar above. The motif logos generated from motifstack are shown above using the color conventions of the genomic sequence below. Predicted transcription factor name and change score (Alterscore-Refscore) are shown to the right of each motif, and the transcription factor with the strongest score is highlighted in red font. Mutations leading to predicted disruption of transcription factor binding have negative change scores, while those creating new transcription factor binding sites will have a positive change scores.