Table 1.
The number and mean of percentage ± standard error of the mean (SEM) of oocytes, fertilized oocytes, embryos in different stages and cleavage-arrested embryos (CAE) with three different types in control and experimental groups
| Groups | Oocytes | Fertilized oocytes | Two-cell | Four-cell | Morulae | Blastocysts | Cleavage-arrested embryos (CAE) | CAE (type I) | CAE (type II) | CAE (type III) |
|---|---|---|---|---|---|---|---|---|---|---|
| Control | 219 | 200 (91.32 ± 1.2) | 158 (79 ± 2.88) | 121 (76.58 ± 2.8) | 96 (79.33 ± 2.79) | 74 (77.08 ± 10.29) | 126 (63 ± 7.1) | 2 (1.58 ± 0.87) | 9 (7.14 ± 0.79) | 116 (92.06 ± 1.5) |
| HSP60 (10 ng/mL) | 219 | 207 (94.52 ± 0.79) | 182 (87.92 ± 1.38)a | 163 (89.56 ± 0.8) | 142 (87.11 ± 2.56) | 122 (85.91 ± 3.96) | 85 (41.06 ± 5.79)a | 5 (5.88 ± 2.94) | 8 (9.41 ± 1.9) | 72 (84.7 ± 5.98) |
| HSP60 (50 ng/mL) | 219 | 187 (85.38 ± 2.77)b | 129 (68.98 ± 1.5)a, b | 91 (61.07 ± 2.7) | 81 (89.01 ± 2.7) | 65 (80.24 ± 0.48) | 122 (65.24 ± 2.13)b | 11 (9.01 ± 1.1) | 18 (14.75 ± 2.25) | 93 (76.22 ± 3.32) |
| HSP60 (100 ng/mL) | 219 | 154 (70.31 ± 3.56)a, b, c | 97 (62.98 ± 1.87)a, b | 76 (78.35 ± 4.38) | 51 (67.1 ± 6.62) | 36 (70.58 ± 3.96) | 118 (76.62 ± 5.73)a, b | 21 (17.79 ± 4.57)a, b | 20 (16.94 ± 1.03)a, b | 77 (65.25 ± 5.46)a, b |
All data is drawn from three replicates.
- Superscript (a) in each column indicates significant difference (P≤0.05) with control group.
- Superscript (b) in each column indicates significant difference (P≤0.05) with HSP60 (10 ng/mL) group.
- Superscript (c) in each column indicates significant difference (P≤0.05) with HSP60 (50 ng/mL) group.