Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (mouse) | CB-17 SCID | Charles River Laboratories | ||
| Strain, strain background (mouse) | C57BL/6 | Charles River Laboratories | ||
| Cell line (human) | KMS11 | Genentech | RRID:CVCL_2989 | Multiple myeloma |
| Cell line (human) | OPM2 | Genentech | RRID:CVCL_1625 | Multiple myeloma |
| Cell line (human) | JJN3 | Genentech | RRID:CVCL_2078 | Multiple myeloma |
| Cell line (human) | RPMI-8226 | Genentech | RRID:CVCL_0014 | Multiple myeloma |
| Cell line (human) | LP-1 | Genentech | RRID:CVCL_0012 | Multiple myeloma |
| Cell line (human) | U266B1 | Genentech | RRID:CVCL_0566 | Multiple myeloma |
| Cell line (human) | Ramos | Genentech | RRID:CVCL_0597 | Burkitt’s lymphoma |
| Cell line (human) | Raji | Genentech | RRID:CVCL_0511 | Burkitt’s lymphoma |
| Cell line (human) | BJAB | Genentech | RRID:CVCL_5711 | Burkitt’s lymphoma |
| Cell line (human) | Daudi | Genentech | RRID:CVCL_0008 | Burkitt’s lymphoma |
| Cell line (human) | Maver-1 | Genentech | RRID:CVCL_1831 | Mantle Cell Lymphoma |
| Cell line (human) | Jurkat | Genentech | RRID:CVCL_0367 | Acute T-Cell Leukemia |
| Cell line (human) | MDA-MB-231 | Genentech | RRID:CVCL_0062 | Triple negative breast cancer |
| Cell line (mouse) | BW5147.3 | Genentech | RRID:CVCL_4135 | Thymic Lymphoma |
| Cell line (mouse) | ABE8.1/2 | Genentech | RRID:CVCL_3487 | Pre-B Cell Lymphoma |
| Cell line (human) | BAX knockout Clone one and Clone 2 | This paper | Isolated clones of CRISPR/Cas9 deletion of BAX gene in KMS11 | |
| Cell line (human) | IRE1 knockout KMS11 | Genentech | Harnoss et al., 2019 | |
| Biological sample (mouse) | BMDC | This paper | Isolated from the tibia and femur bones of C57BL/6 mice | |
| Transfected construct (human) | LDTM (1-470) | This paper | IRE1 aa1-470 with or without C-terminal Flag tag under CMV promoter | |
| Transfected construct (human) | 1-507/1-507F | This paper | IRE1 aa1-507 with or without C-terminal Flag tag under CMV promoter | |
| Transfected construct (human) | IRE1 shRNA | Genentech | Harnoss et al., 2019 | |
| Transfected construct (human) | IRE1 wt | This paper | Full-length wild-type IRE1 expressed from CMV promoter | |
| Transfected construct (human) | IRE1 D507A | This paper | Asp at 507 was mutated to Ala | |
| Transfected construct (human) | IRE1 D512A | This paper | Asp at 512 was mutated to Ala | |
| Transfected construct (human) | IRE1 D507A, D512A | This paper | Both Asp at 507 and 512 were mutated to Ala | |
| Transfected construct (human) | IRE1 LKR | This paper | IRE1 aa468-977 with N-terminal His tag expressed from CMV promoter | |
| Transfected construct (human) | BAX gRNA_1 | This paper | GCGGTGATGGACGGGTCCG | |
| Transfected construct (human) | BAX gRNA_2 | This paper | TTCATGATCTGCTCAGAGC | |
| Antibody | GAPDH-HRP | Cell Signaling Technology | Cat. #: 2118 | (1:5000) |
| Antibody | XBP1s (rabbit monoclonal) | Genentech (Chang et al., 2018) |
(1:1000) | |
| Antibody | IRE1α LD (mouse monoclonal, IgG2a) | This study | (1:1000) | |
| Antibody | IRE1α CD (rabbit monoclonal) | Cell Signaling Technology | Cat. #: 3294 | (1:1000) |
| Antibody | BAX (rabbit polyclonal) | Cell Signaling Technology | Cat. #: 2772 | (1:1000) |
| Antibody | Cytochrome-C (rabbit monoclonal) | Cell Signaling Technology | Cat. #: 11940 | (1:1000) |
| Antibody | COXIV (rabbit monoclonal) | Cell Signaling Technology | Cat. #: 4850 | (1:1000) |
| Antibody | ATF4 (rabbit monoclonal) | Cell Signaling Technology | Cat. #: 11815 | (1:1000) |
| Antibody | BiP (rabbit monoclonal) | Cell Signaling Technology | Cat. #: 3177 | (1:1000) |
| Antibody | Cleaved caspase-3 (rabbit monoclonal) | Cell Signaling Technology | Cat. #: 9664 | (1:1000) |
| Antibody | CHOP (mouse monoclonal IgG2a) | Cell Signaling Technology | Cat. #: 2895 | (1:1000) |
| Antibody | Flag (mouse monoclonal IgG1) | Sigma-Aldrich | Cat. #: F1804 | (1:1000) |
| Antibody | Calnexin (rabbit polyclonal) | Abcam | Cat. #: ab22595 | (1 μg/ml) |
| Antibody | Anti-rabbit IgG HRP | Jackson ImmunoResearch Laboratories | Cat. #: 711-035-152 | (1:10,000) |
| Antibody | Anti-mouse IgG2a HRP | SouthernBiotech | Cat. #:1080–05 | (1:10,000) |
| Antibody | pIRE1α (rabbit monoclonal) | Genentech (Chang et al., 2018) | (1:500) | |
| Recombinant DNA reagent | pRK.TK.Neo-IRE1 WT | Genentech | IRE1 wild-type cDNA in pRK.TK.Neo backbone | |
| Recombinant DNA reagent | pRK.TK.Neo-IRE1 D507A | Genentech | IRE1 cDNA with Asp 507 mutated to Ala in pRK.TK.Neo backbone | |
| Recombinant DNA reagent | pRK.TK.Neo-IRE1 D512A | Genentech | IRE1 cDNA with Asp 512 mutated to Ala in pRK.TK.Neo backbone | |
| Recombinant DNA reagent | pRK.TK.Neo-IRE1 D507A, D512A | Genentech | IRE1 cDNA with Asp 507 and 512 mutated to Ala in pRK.TK.Neo backbone | |
| Recombinant DNA reagent | pRK.TK.Neo-IRE1 1–470-Flag | Genentech | IRE1 cDNA aa1-470 with C-terminal Flag in pRK.TK.Neo backbone | |
| Recombinant DNA reagent | pRK.TK.Neo-IRE1 1–470 | Genentech | IRE1 cDNA aa1-470 in pRK.TK.Neo backbone | |
| Recombinant DNA reagent | pRK.TK.Neo-IRE1 1–507-Flag | Genentech | IRE1 cDNA aa1-507 with C-terminal Flag in pRK.TK.Neo backbone | |
| Recombinant DNA reagent | pRK.TK.Neo-IRE1 1–507 | Genentech | IRE1 cDNA aa1-507 in pRK.TK.Neo backbone | |
| Recombinant DNA reagent | pcDNA3.1.Zeo-IRE1 6xHis 468–977 | Genentech | IRE1 cDNA aa468-977 with N-terminal His tag in pcDNA3.1.Zeo backbone | |
| Commercial assay or kit | Caspase-Glo 3/7 Assay | Promega | G8090 | |
| Commercial assay or kit | Caspase-Glo 9 Assay | Promega | G8210 | |
| Commercial assay or kit | CellTiter-Glo Luminescent Cell Viability Assay | Promega | G7570 | |
| Commercial assay or kit | MitoProbe JC-1 Assay Kit for Flow Cytometry | ThermoFisher Scientific | M34152 | |
| Commercial assay or kit | Subcellular Protein Fractionation Kit for Cultured Cells | ThermoFisher Scientific | 78840 | |
| Commercial assay or kit | Mitochondria Isolation Kit for Cultured Cells | ThermoFisher Scientific | 89874 | |
| Recombinant protein | LKR | This paper | Purified N-terminally His-tagged IRE1 aa468-977 | |
| Recombinant protein | Caspase 3 | Enzo Life Sciences | ALX-201–059 | |
| Recombinant protein | Caspase 7 | BioVision | 1087 | |
| Chemical compound, drug | Rhod-2, AM, cell permeant | Invitrogen | R1244 | |
| Chemical compound, drug | Thapsigargin, Tg | Tocris | 1138 | |
| Chemical compound, drug | Tunicamycin, Tm | Tocris | 3516 | |
| Chemical compound, drug | Brefeldin A, BfeA | Tocris | 1231 | |
| Chemical compound, drug | DTT | ThermoFisher Scientific | R0861 | |
| Chemical compound, drug | Cycloheximide, CHX | Sigma-Aldrich | C4859 | |
| Chemical compound, drug | Z-VAD-FMK, zVAD | R and D Systems | FMK001 | |
| Chemical compound, drug | Doxycycline, DOX | Clontech | NC0424034 | |
| Chemical compound, drug | ABT-199 | Genentech | G00376771 | |
| Chemical compound, drug | Subtilase toxin AB5, SubAB | Paton et al., 2006 | ||
| Software, algorithm | Prism 7 | GraphPad | ||
| Software, algorithm | ImageJ | NIH | ||
| Software, algorithm | FlowJo 10.4 | FlowJo, LLC |