Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Peptide, recombinant protein | AcrVA4 | Watters et al., 2018 | Addgene #115656 | |
| Peptide, recombinant protein | LbCas12a | Watters et al., 2018 | Addgene #115656 | |
| Peptide, recombinant protein | AsCas12a | Watters et al., 2018 | Addgene #113430 | |
| Strain, strain background (E. coli) | NEB 10-beta | New England Biolabs | ||
| Strain, strain background (E. coli) | MG1655 λ+(cI857 bor::kanR) | doi: 10.1073/pnas.0808831105 | ||
| Strain, strain background (E. coli) | Rosetta 2 (DE3) | Novagen | ||
| Strain, strain background (lambda) | λ phage | This paper | cI857 bor::kanR | |
| Sequence-based reagent | LbCas12a pre-crRNA | IDT | lab archive:rGJK_006 | rArGrArUrUrArArArUrArArUrUrUrCrUrArCrUrArArGrUrGrUrArGrArUrArArArGrUrGrCrUrCrArUrCrArUrUrGrGrArArArArCrGrU |
| Sequence-based reagent | AsCas12a pre-crRNA | IDT | lab archive:rGJK_008 | rGrArCrCrUrUrUrUrUrArArUrUrUrCrUrArCrUrCrUrUrGrUrArGrArUrArArArGrUrGrCrUrCrArUrCrArUrUrGrGrArArArArCrGrU |
| Sequence-based reagent | LbCas12a crRNA | IDT | lab archive:rGJK_017 | rArArUrUrUrCrUrArCrUrArArGrUrGrUrArGrArUrGrArUrCrGrUrUrArCrGrCrUrArArCrUrArUrGrA |
| Sequence-based reagent | AsCas12a crRNA | IDT | lab archive:rGJK_018 | rArArUrUrUrCrUrArCrUrCrUrUrGrUrArGrArUrGrArUrCrGrUrUrArCrGrCrUrArArCrUrArUrGrA |
| Sequence-based reagent | Non-target DNA strand | IDT | lab archive:dGJK_006 | GAC GAC AAA ACT TTA GAT CGT TAC GCT AAC TAT GAG GGC TGT CTG TGG AAT GCT A |
| Sequence-based reagent | Target DNA strand | IDT | lab archive:dGJK_007 | TAG CAT TCC ACA GAC AGC CCT CAT AGT TAG CGT AAC GAT CTA AAG TTT TGT CGT C |
| Sequence-based reagent | LbCas12a crRNA (dT −21) | IDT | lab archive:rGJK_051 | rArGrArUrUrArArATrArArUrUrUrCrUrArCrUrArArGrUrGrUrArGrArUrArArArGrUrGrCrUrCrArUrCrArUrUrGrGrArArArArCrGrU |
| Sequence-based reagent | LbCas12a crRNA (5'-PO4) | IDT | lab archive:rGJK_053 | /5Phos/rArArUrUrUrCrUrArCrUrArArGrUrGrUrArGrArUrArArArGrUrGrCrUrCrArUrCrArUrUrGrGrArArArArCrGrU |
| Software, algorithm | Prism7 | GraphPad | ||
| Software, algorithm | ImageQuantTL | GE Healthcare | ||
| Software,algorithm | ScÅtter 3.0 | BioIsis |