Table 1. Cryo-EM data collection, reconstruction, and model statistics.
| State I | State II | |
|---|---|---|
| PDB: 6P7M | EMDB: 20266 | PDB: 6P7N | EMDB: 20267 | |
| Data Collection | ||
| Microscope | FEI Titan Krios | FEI Titan Krios |
| Voltage (kV) | 300 | 300 |
| Camera | Gatan K2 Summit | Gatan K2 Summit |
| Defocus range (µm) | 0.7 ~ 2.2 | 0.7 ~ 2.2 |
| Pixel Size (Å) | 0.9 | 0.9 |
| Magnification | 135000 | 135000 |
| Electron Dose (e/Å2) | 47 | 47 |
| Total Particles | 324336 | 324336 |
| Reconstruction | ||
| Software | CryoSparc | CryoSparc |
| Symmetry Imposed | C1 | C1 |
| Final number of refined particles | 156979 | 79787 |
| Resolution of polished unmasked map (Å) | 4.2 | 8.7 |
| Resolution of polished masked map (Å) | 3 | 5 |
| Map Sharpening B-factor (Å2) | −101.4 | −123.5 |
| Refinement | ||
| Model Resolution cutoff (Å) | 3 | 5 |
| FSC threshold | 0.143 | 0.143 |
| Map CC (box) | 0.74 | 0.78 |
| Map CC (mask) | 0.79 | 0.51 |
| R..m.s Deviations | ||
| Bond lengths (Å) | 0.006 | 0.002 |
| Bond angles (°) | 0.607 | 0.524 |
| Molprobity score | 1.77 | 1.84 |
| Clashscore | 7.87 | 6.63 |
| Rotamer Outliers (%) | 0 | 1.33 |
| Ramachandran plot | ||
| Favored (%) | 94.43 | 94.43 |
| Allowed(%) | 5.57 | 5.57 |
| Outliers (%) | 0 | 0 |