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. 2019 Aug 23;12:1473–1492. doi: 10.2147/DMSO.S214093

Figure 1.

Figure 1

HF feeding-induced obesity is associated with an impairment of energy expenditure that is age-dependent. (A) A schema for the animal experiments. C57BL/6NCrSlc male mice were fed with a control (CRF) diet or a high-fat diet (HFD) for 12 or 24 weeks. 24w/C/HF group mice were fed for 12 weeks with the CRF diet and then switched to an HFD for 12 weeks. All mice started their feeding experiments at 6 weeks of age (each group; N=7). Metabolic function tests were performed in the final week (★; time metabolic function tests were conducted). After feeding for 12 or 24 weeks, mice were sacrificed and organs were collected (x; time of euthanasia.) (B) Body mass was measured once weekly and (C) Energy expenditure (EE; kcal/kg/h), Integrated EE for each 16 h period, (D) Glucose tolerance testing, (E) Voluntary exercise (number of rotations per day) were measured in a voluntary wheel running chamber system were measured within a week of the end-point. Values are mean ± SD. **p<0.001, vs 12w/C. ††p<0.001, vs 12w/HF. There are no significant differences between 24w/HF and 24w/C/HF groups.