Table 1.
List of primer sequences used in qPCR assays
| Gene | Primer sequence 5ʹ to 3’ | Product size | |
|---|---|---|---|
| ACC1 | Forward | GTCTGCTGGGAAGTTAATCCAG | 432bp |
| Reverse | TCCTGCAGCTCTAGCAGAGG | ||
| ACOX | Forward | GCCAGGACTATCGCATGATT | 73bp |
| Reverse | GCCCAACTGTGACTTCCATC | ||
| CPT1 | Forward | TCCATGCATACCAAAGTGGA | 185bp |
| Reverse | TGGTAGGAGAGCAGCACCTT | ||
| CPT2 | Forward | GCCCAGCTTCCATCTTTACT | 254bp |
| Reverse | CAGGATGTTGTGGTTTATCCGC | ||
| FAS | Forward | TACCAGTGCCACAGGAGTCTCA | 175bp |
| Reverse | CGGGTGAGGACGTTTACAAAG | ||
| GAPDH | Forward | AGTGTTTCCTCGTCCCGTAG | 110bp |
| Reverse | CAATCTCCACTTTGCCACTG | ||
| MCP1 | Forward | ACCTGGATCGGAACCAAATG | 148bp |
| Reverse | GTGCTTGAGGTGGTTGTGGA | ||
| p16 | Forward | CCCAACGCCCCGAACT | 63bp |
| Reverse | GTGAACGTTGCCCATCATCA | ||
| p21 | Forward | GCAAAGTGTGCCGTTGTCTC | 112bp |
| Reverse | TCAAAGTTCCACCGTTCTCG | ||
| PAI1 | Forward | TTGTGGAACTGCCCTACCAG | 125bp |
| Reverse | GCCCTTCCATTGTCTGATGA | ||
| PGC1a | Forward | TCCTCTGACCCCAGAGTCAC | 140bp |
| Reverse | CTTGGTTGGCTTTATGAGGAGG | ||
| PPARα | Forward | CAGAGCAACCATCCAGATGACACC | 121bp |
| Reverse | CGGACTCGGTCTTCTTGATGACCT | ||
| PPARγ | Forward | ATGGGTGAAACTCTGGGAGA | 75bp |
| Reverse | GCTCATAGGCAGTGCATCAG | ||
| SREBP-1c | Forward | GCGCTACCGGTCTTCTATCA | 113bp |
| Reverse | GGATGTAGTCGATGGCCTTG | ||
| TGFb1 | Forward | ACAATTCCTGGCGTTACCTT | 123bp |
| Reverse | AGCCCTGTATTCCGTCTCC | ||
| TNFa | Forward | AACCTCCTCTCTGCCGTCAA | 170bp |
| Reverse | CCGGACTCCGCAAAGTCTAA | ||
| UCP1 | Forward | TCTCTTCAGGGAGAGAAACACC | 83bp |
| Reverse | AATGAACACTGCCACACCTC |
Abbreviations: ACC1, acetyl-CoA carboxylase 1; ACOX, Acyl-Coenzyme A Oxidase; CPT1, carnitine palmitoyltransferase 1; CPT2, carnitine palmitoyltransferase 2; FAS, fatty acid synthase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MCP1, monocyte chemotactic protein-1; PAI1, plasminogen activator inhibitor-1; PGC-1α, peroxisome proliferator-activated receptor γ co-activator 1α; PPARα, peroxisome proliferator-activated receptor α; PPARγ, peroxisome proliferator-activated receptor γ; SREBP1c, sterol regulatory element-binding protein 1c; TGFb1, transforming growth factor beta 1; TNFα, tumor necrosis factor alpha; UCP1, uncoupling protein 1.