Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Braf | Ensembl: ENSMUSG00000002413 |
||
| Gene (Mus musculus) | Pik3ca | Ensembl: ENSMUSG00000027665 |
||
| Genetic reagent (Mus musculus) | BrafCA | McMahon lab stock | MGI:Braftm1Mmcm
JAX:017837 RRID:IMSR_JAX:017837 |
|
| Genetic reagent (Mus musculus) | Pik3caH1047R | Wayne Phillips | MGI:Pik3catm1.1Waph
RRID:MGI:5427584 |
|
| Genetic reagent (Mus musculus) | BrafCAT | This paper | N/A | New genetically engineered mouse reported in this paper |
| Genetic reagent (Mus musculus) | KrasLSL | The Jackson Lab | MGI:Krastm4Tyj
JAX:008179 RRID:IMSR_JAX:008179 |
|
| Cell line (Mus musculus) | MLE-12 | ATCC | CRL-2110 RRID:CVCL_3751 |
|
| Cell line (Homo sapiens) | 293T | Lab Stock | RRID:CVCL_0063 | |
| Antibody | Mouse monoclonal PGC1α | Millipore | Cat# 1F3.9 RRID:AB_10806332 |
Co-IP, 10 ug WB, 1:500 |
| Antibody | Rabbit polyclonal PGC1α | Millipore | Cat# AB3242 RRID:AB_2268462 |
IHC, 1:50 WB, 1:500 |
| Antibody | Rabbit monoclonal NKX2-1 | Abcam | Cat# AB76013 RRID:AB_1310784 |
Co-IP, 10 ug IHC, 1:250 WB, 1:1000 |
| Antibody | Rabbit polyclonal Phospho-S327-NKX2-1 | CST | Cat# 13608 RRID:AB_2798273 |
IHC, 1:250 |
| Antibody | Rabbit monoclonal FOXA1 | Abcam | Cat# AB23738 RRID:AB_2104842 |
IHC, 1:250 |
| Antibody | Rabbit monoclonal FOXA1 | CST | Cat# 58613 RRID:AB_2799548 |
WB, 1:2500 |
| Antibody | Rabbit monoclonal FOXA2 | CST | Cat# D56D6 RRID:AB_10891055 |
IHC, 1:250 WB, 1:1000 |
| Antibody | Rabbit monoclonal SFTPA1 + 2 | Abcam | Cat# AB206299 RRID:AB_2810211 |
IHC, 1:250 WB, 1:1000 |
| Antibody | Goat polyclonal SFTPA | Santa Cruz | Cat# SC-7699 RRID:AB_661292 |
IHC, 1:100 WB, 1:1000 |
| Antibody | Goat polyclonal SFTPA | Santa Cruz | Cat# SC-7700 RRID:AB_661293 |
IHC, 1:100 WB, 1:1000 |
| Antibody | Rabbit polyclonal SFTPA | Santa Cruz | Cat# SC-13977 RRID:AB_661294 |
WB, 1:1000 |
| Antibody | Rabbit monoclonal Lysozyme | Abcam | Cat# AB108508 RRID:AB_10861277 |
IHC, 1:250 WB, 1:1000 |
| Antibody | Goat polyclonal SFTPC | Santa Cruz | Cat# SC-7705 RRID:AB_2185505 |
IHC, 1:250 WB, 1:1000 |
| Antibody | Rabbit polyclonal SFTPC | Santa Cruz | Cat# SC-13979 RRID:AB_2185502 |
WB, 1:1000 |
| Antibody | Goat polyclonal CCA | Santa Cruz | Cat# SC-9772 RRID:AB_2238819 |
IHC, 1:1000 |
| Antibody | Goat polyclonal AQP5 | Santa Cruz | Cat# SC-9890 RRID:AB_2059877 |
IHC, 1:50 |
| Antibody | Rabbit polyclonal NR5A2 | Abcam | Cat# AB153944 RRID:AB_2810212 |
WB, 1:1000 |
| Recombinant DNA reagent | M50 Super 8x TOPFlash | Addgene | Plasmid #12456 RRID:Addgene_12456 |
Vector used to build SFTP Luciferase reporters |
| Recombinant DNA reagent | pCDNA3-mLRH1 | Holly Ingraham | Ms NR5A2 expression vector | |
| Recombinant DNA reagent | pDTA-TK | Addgene | Plasmid #22677 RRID:Addgene_22677 |
Empty targeting vector for mouse production |
| Recombinant DNA reagent | MSCV-NKX2.1 | Addgene | Plasmid #31271 RRID:Addgene_31271 |
NKX2.1 expression vector |
| Recombinant DNA reagent | PCDH-FOXA1 | Eric Snyder | FOXA1 expression vector | |
| Recombinant DNA reagent | FUW-mKate | This paper | Fluorescent protein mKate expression vector used for assaying transfection efficiency | |
| Recombinant DNA reagent | GFP-PGC1 | Addgene | Plasmid #4 RRID:Addgene_4 |
PGC1α expression vector |
| Recombinant DNA reagent | pWZL-Hygro | Addgene | Plasmid #18750 RRID:Addgene_18750 |
Empty vector used to normalize amount of DNA transfected |
| Recombinant DNA reagent | SFTPA-LUC | This paper | 5 kb SFTPA promoter in luciferase reporter | |
| Recombinant DNA reagent | SFTPB-LUC | This paper | 5 kb SFTPB promoter in luciferase reporter | |
| Recombinant DNA reagent | SFTPC-LUC | This paper | 5 kb SFTPC promoter in luciferase reporter | |
| Peptide, recombinant protein | TAT-CRE | Excellgen | Cat# Eg-1001 | Cell permeant CRE protein |
| Commercial assay or kit | Dynabeads protein G IP kit. | Thermo | Cat# 10007D | |
| Commercial assay or kit | Pierce firefly luc one step glow assay kit | Thermo | Cat# 16196 | |
| Software, algorithm | MEME | http://meme-suite.org | Multiple Em Motif Elucidation | |
| Software, algorithm | FIMO | http://meme-suite.org | Find Individual MOtifs | |
| Software, algorithm | GSEA | http://gsea.org | Gene Set Enrichment Analysis | |
| Software, algorithm | R | http://rstudio.com | R programming language | |
| Software, algorithm | Custom R scripts |
github.com/jevanveen
/vanveen-elife |
Referenced scripts hosted at GitHub | |
| Other | Antigen Retrieval | Syrbu and Cohen, 2011 | Antigen Retrieval Method for Immunostaining of Paraffin Sections | Method greatly aiding in immunostaining |