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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Cytometry A. 2019 Mar 5;95(4):442–449. doi: 10.1002/cyto.a.23737

Figure 2. Reduced CD40-mediated NFκB p65 phosphorylation in GC B cells despite increased surface CD40 expression.

Figure 2.

NFκB p65 phosphorylation (p-p65) at serine 529 following 15 min CD40L stimulation was measured by mass cytometry. Populations were gated on biaxial plots; GC B cells as CD3−CD19+CD20++CD38+ and non-GC B cells as CD3−CD19+CD38−. The non-GC population was further gated to enrich for IgD+ naïve B cells (possibly containing unswitched memory B cells) and IgD− memory B cells. A) Histograms of p-p65 from one representative tonsil (T19). B-C) Relative induction of p-p65 by CD40L (B) and surface CD40 expression in unstimulated cells (C) was measured simultaneously. Mean ± SD. * p < 0.05; repeated measurement one-way ANOVA and Tukey’s multiple comparison test.