Table 1b.
Primary and secondary antibodies, and recombinant proteins.
| Antibody | Species | Type | Dilution | Source |
|---|---|---|---|---|
| Alexa Fluor® Goat anti-mouse IgG 488/594 | Goat | Polyclonal | 1:200 (IF) | Invitrogen, Karlsruhe, Germany |
| Alexa Fluor® Goat anti-rabbit IgG 488/594 | Goat | Polyclonal | 1:200 (IF) | Invitrogen, Karlsruhe, Germany |
| Alexa Fluor® Donkey anti-goat IgG 594 | Donkey | Polyclonal | 1:200 (IF) | Invitrogen, Karlsruhe, Germany |
| beta-III-tubulin | Mouse | Monoclonal | 1:100 (IF) | Merck Millipore, Darmstadt, Germany |
| PGP9.5 | Mouse | Monoclonal | 1: 2000 (IHC), 1:200 (IF) | DAKO, Hamburg, Germany |
| CGRP | Rabbit | Polyclonal | 1:150 (IF), 1:200 (IHC) | Merck Millipore, Darmstadt, Germany |
| Substance P | Mouse | Monoclonal | 1:30 (IF), 1:150 (IHC) | Santa Cruz, Dallas, TX, USA |
| VIP | Rabbit | Polyclonal | 1:80 (IF), 1:500 (IHC) | Sigma-Aldrich, St. Louis, MO, USA |
| nNOS | Rabbit | Polyclonal | 1:500 (IHC) | Cell Signaling, Cambridge, UK |
| nNOS | Goat | Polyclonal | 1:500 (IF) | CalBiochem (San Diego, CA, USA) |
| BDNF | Mouse | Monoclonal | 10 μg/ml (N) | CalBiochem (San Diego, CA, USA) |
| NT-3 | Mouse | Monoclonal | 1 μg/ml (N) | R&D systems, Germany |
| NGF | Rabbit | Polyclonal | 3 μg/ml (N) | Acris Antibodies, Herford, Germany |
| S100 | Mouse | Monoclonal | 1:150 (IF) | Merck Millipore, Darmstadt, Germany |
| Recombinant protein | ||||
| rhBDNF | Human | 10 ng/ml | Sigma-Aldrich, St. Louis, MO, USA |
IHC: immunohistochemistry, IF: immunofluorescence, N: neutralization, rh: recombinant human.