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. 2019 Jun;11(3):225–231.

Table 1.

Primers and conditions of polymerase chain reaction in K. pneumoniae

Primer Name Primer Sequence (5′ to 3′) Cycle Initial Denaturati Cycling Final Extension Length (bp)
IMP-1 F: ACCGCAGCAGAGTCTTTGCC
R: ACAACCAGTTTTGCCTTACC
33 94°C 5 min 94°C 30 s, 56°C 30 s, 72°C 1 min 72°C, 7 min 587 bp
VIM F: CAAGTCCGTTAGCCCATTCC
R: GGCACAACCACCGTATAGCAC
33 94°C 5 min 94°C 30 s, 57°C 30 s, 72°C 1 min 72°C, 10 min 539 bp
VIM2 F: ATTGGTCTATTTGACCGCGTC
R: TGCTACTCAACGACTGAGCG
33 94°C 5 min 94°C 30 s, 56°C 30 s,72 °C 1 min 72°C, 10 min 780 bp