Fig. 6.

TDP1 and DJ-1 localization in nucleoids in mitochondria in ATII cells. Lung tissue was obtained from non-smokers (NS), smokers (SM) and emphysema patients (EM). A - TDP1 expression (red) was analyzed in ATII cells identified using SP-A antibody (violet) in lung tissue sections by immunohistofluorescence. MtDNA was detected by Picogreen (green). B – Relative TDP1 fluorescence intensity was determined in mitochondrial nucleoids in ATII cells. C - TDP1 binding to mtDNA was determined in lung tissue by CHIP assay. D - DJ-1 expression (red) was analyzed in mtDNA (green) in ATII cells (violet) by immunohistofluorescence. E - Relative DJ-1 fluorescence intensity in mitochondrial nucleoids in ATII cells is also shown. F – DJ-1 binding to mtDNA was analyzed in lung tissue by CHIP assay. Data is expressed as means ± s.e.m., N = 3 per group, *P < .05, **P < .001.