Fig. 6.

MiR-134 overexpression suppresses the proliferation and promotes the apoptosis of ESCC cells. ESCC cells were treated with miR-134 expression and/or si-PLXNA1. (a) the proliferation of ESCC cells assessed by CCK-8 assay and analysed by repeated measures ANOVA; (b) a flow chart depicting cell cycle distribution in ESCC cells as assessed by flow cytometry; (c), the cell proportion/ratio in each cell-cycle phase; (d) the flow cytometry scatter plot of cell apoptosis in ESCC cells; (e) the ESCC cell apoptosis rate determined by Annexin V/PI double staining. ^⁎p < .05 vs. the blank and NC groups (cells without transfection or transfected with empty plasmid); ^#p < .05 vs. the miR-134 and si-PLXNA1 groups (cells transfected with miR-134 or si-PLXNA1). Measurement data were expressed as mean ± standard error of mean and analysed by one-way ANOVA, followed by Tukey's post hoc test; the experiment was repeated three times. ESCC, esophageal squamous cell carcinoma; miR-134, microRNA-134; ANOVA, analysis of variance; PLXNA1, plexin A1; NC, negative control; OD, optical density.