The expression and function of TWIST1 in NSCLC cell lines. a, The mRNA expression levels of TWIST1 in four NSCLC cell lines A549, H1975, H1299, and H358. b and c, The mRNA and protein expression of TWIST1 in control cells and TWIST1-OE H1975 cells. d and e, Scratch test and transwell migration test in control cells and TWIST1-OE H1975 cells. All of the data are expressed as mean values ± SEM (n = 3); ⁎P < .05, ⁎⁎P < .01, ⁎⁎⁎P < .001 (Student's t-test).