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Performance characteristics of hepatitis A virus triplex and 5′-UTR rRT-PCR assays
| Assay | Performance characteristic |
|||
|---|---|---|---|---|
| LOD (no. of copies/reaction) | Accuracy (%) | Sensitivity (%) | Specificity (%) | |
| Triplex IA | 1 | 100 | 100 | 100 |
| Triplex IB | 10 | 99.2 | 97.8 | 100 |
| Triplex IIIA | 10 | 100a | 100 | 100a |
| 5′-UTR | 1 | 98.4a | 98.8a | 97.3a |
Performance characteristic determined after discrepancy resolution.
