TABLE 2.
Allele-specific real-time PCR assays developed in this study
| Assay name | Nucleotide change | Amino acid substitution |
|---|---|---|
| CYP51A_rfa | NAb | NA |
| CYP51A_TR34_mu | −322 to −288 insertion | NA |
| CYP51A_TR46_mu | −331 to −285 insertion | NA |
| CYP51A_G54R_mu | G160C | G54R |
| CYP51A_G54W_mu | G160T | G54W |
| CYP51A_L98H_mu | T293A | L98H |
| CYP51A_Y121F_mu | A362T | Y121F |
| CYP51A_M220I_mu | G731T | M220I |
a
CYP51A_rf is a real-time quantitative PCR assay targeting a mutation-free region of cyp51A to further quantify the total DNA of A. fumigatus in the mixture of triazole-susceptible and -resistant strains.
b
NA, not applicable.