TABLE 3.
Primers, probes, and blockers used in this study
| Assay | Primer/probe/blockera | Sequence (5′–3′) |
|---|---|---|
| CYP51A_rf | cyp51_F | CGGCCGGATGGACATCT |
| cyp51_R | GCTCGAGCAGCGGTAAAAAT | |
| cyp51_LST | Fam-CAATGGCTGAGATTAC-MGB | |
| CYP51A_TR34_mu | TR34_ASP | GAGCCGAATGAATCAC |
| TR_LST | Fam-TTCCAGCATACCATACAC-MGB | |
| TR_LSP | ACCAATATAGGTTCATAGGTAAGTAGATCTACC | |
| CYP51A_TR46_mu | TR46_ASP | CCGAATGAAAGTTGTCTAGAA |
| TR_LST | Fam-TTCCAGCATACCATACAC-MGB | |
| TR_LSP | ACCAATATAGGTTCATAGGTAAGTAGATCTACC | |
| CYP51A_G54R_mu | G54R_ASP | CTGGGTAGTACCATCAGTTACC |
| G54_ASB | GGTAGTACCATCAGTTACGGG-MGB | |
| G54_LST | Fam-TTGATCCCTACAAGTTCTT-MGB | |
| G54_LSP | GTCAAACTACAATCTTGAGACTTGCCT | |
| CYP51A_G54W_mu | G54W_ASP | CTGGGTAGTACCATCAGTTACT |
| G54_ASB | GGTAGTACCATCAGTTACGGG-MGB | |
| G54_LST | Fam-TTGATCCCTACAAGTTCTT-MGB | |
| G54_LSP | GTCAAACTACAATCTTGAGACTTGCCT | |
| CYP51A_L98H_mu | L98H_ASP | CTCAACGGCAAGCA |
| L98H_ASB | AACGGCAAGCTCA-MGB | |
| L98H_LST | Fam-TAGTCCATTGACGACCC-MGB | |
| L98H_LSP | ACGTCCGATCCGAAAACG | |
| CYP51A_Y121F_mu | Y121F_ASP | CGGATCGGACGTGGTGTT |
| Y121F_ASB | ATCGGACGTGGTGTATG-MGB | |
| Y121F_LST | Fam-CAGAAAAAGTTCATCAAGTAC-MGB | |
| Y121F_LSP | GCAGATAGTCCAAAACCTCCTTCT | |
| CYP51A_M220I_mu | M220I_ASP | TGGGGCCCACGGTAGA |
| M220I_ASB | GGCCCACGGTAGCAT-MGB | |
| M220I_LST | Fam-AAGCCCTTGTCCAGGTC-MGB | |
| M220I_LSP | CAAGGCCAGGAAGTTCGTTC | |
a
ASP, allele-specific primer; ASB, allele-specific blocker; LST, locus-specific TaqMan probe; LSP, locus-specific primer.