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. 2019 Jul 29;24(9):812–825. doi: 10.1007/s10495-019-01560-w

Fig. 1.

Fig. 1

Effects of clinorotation for 48 h on autophagy level in HUVECs. a Western blot analysis of LC3II and p62 in HUVECs exposed to clinorotation for 48 h with or without BafA1 (20 nM) or treatment with rapamycin (1 μM) alone. b Immunostaining of endogenous LC3 puncta (green) and nuclei (blue) in HUVECs and the quantification of endogenous LC3 puncta per cell. The data are expressed as the mean ± SD of three replicates each. *p < 0.05 vs. the control. Scale bar: 20 μm