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. 2019 Jul 29;24(9):812–825. doi: 10.1007/s10495-019-01560-w

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Effects of 3-MA on apoptosis rate after clinorotation for 48 h in HUVECs. a Western blot analysis of Bcl-2 and cleaved caspase 3 after clinorotation for 48 h with or without 3-MA (5 mM). b Annexin V-FITC/PI staining analyzed by flow cytometry for the percentage of apoptosis in HUVECs after 48 h clinorotation with or without 3-MA (5 mM). The data are expressed as the mean ± SD of three replicates.*p < 0.05 vs. the control