Fig. 6.

Effects of BafA1 on the UPR and ER stress-mediated apoptosis in HUVECs under clinorotation. a Western blot analysis of p-PERK (Thr980), p-EIF2S1 (Ser51), CHOP, ATF6, and GRP78, along with Bcl-2 and cleaved caspase 3 in HUVECs under 48 h clinorotation in the presence or absence of BafA1 (20 nM). b Annexin V-FITC/PI staining for the apoptosis rates in HUVECs analyzed by flow cytometry under 48 h clinorotation in the presence or absence of BafA1 (20 nM). c Immunostaining of endogenous LC3 puncta (green), ubiquitin (red), and nuclei (blue) in HUVECs under 48 h clinorotation in the presence or absence of BafA1 (20 nM). The data are expressed as the mean ± SD of three replicates each. *p < 0.05 vs. the control. Scale bar: 20 μm