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. 2019 Aug 27;10:3868. doi: 10.1038/s41467-019-11740-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Mutational analysis of His1808. Wild-type or mutant NocTE was incubated with the SNAC thioester of the epi-nocardicin G 5 and monitored by HPLC for its ability to catalyze epimerization and hydrolysis to 3 or hydrolysis to 6. Chromatograms for assays were monitored at 272 nm. Mutation of His1808 had little impact on catalytic activity, but the fraction of 6 in the product rises from ≤1% in wild type to ~15% in the H1808A mutant