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. 2019 Aug 27;5:131. doi: 10.1038/s41420-019-0211-5

Fig. 3. Effects of p53DN on transcription activity and apoptosis in LNCaP cells in response to DOX, CPT and BTZ.

Fig. 3

a Comparison of protein levels of p53, p21 and caspase 3(a) and GAPDH between LNCaP–EGFP (EGFP) and LNCaP–p53DN (p53DN) cells treated with 0, 0.5, and 1 μM of DOX for 24 h, analyzed by immunoblotting. b Comparison of protein levels of p53, p21 and caspase 3(a) and GAPDH between LNCaP–EGFP and LNCaP–p53DN cells treated by 0, 1, and 3 μM of CPT for 24 h, analyzed by immunoblotting. c Comparison of protein levels of p53, p21 and caspase 3(a) and GAPDH between LNCaP–EGFP and LNCaP–p53DN cells treated with 0, 0.025, 0.05, and 0.1 μM of BTZ for 36 h, analyzed by immunoblotting. Quantitative results of immunoblot image are shown on the right side. **p < 0.01 vs control