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. 2019 Aug 27;9:209. doi: 10.1038/s41398-019-0529-z

Fig. 3. ST8SIA3 deficiency affected the overall sialylation of the striatal glycoproteins.

Fig. 3

a, b The quantitative analysis of a glycan microarray for anti-diSia-Gal antibody S2-566 and anti-triSia antibody A2B5 immunoreactivity is shown. Images of the slide obtained from a fluorescence scan after antibody incubation. The grid contains 20 types of glycans listed in Supplementary Table 2. n = 10 for each glycans with duplicates. c, d Disialyl and trisialyl units were detected in the striatal lysate. Immunoblotting revealed a significant reduction in the levels of the diSia-Gal and triSia terminal glycotopes in the striatum of St8sia3-KO (−/−) mice compared with WT (+/+) control. n = 3 for each genotype. e, f Immunoreactivity for the oligoSia and polySia-conjugated glycoproteins was detected using an anti-oligoSia antibody 12E3 and anti-polySia antibody PSA. Similar levels of the oligo-sialylated and poly-sialylated terminal glycotopes were observed in the striatum of WT and St8sia3-KO mice. n = 3 for each genotype. ACTIN was used as an internal loading control