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. 2019 Aug 27;9:209. doi: 10.1038/s41398-019-0529-z

Fig. 4. Genetic ablation of St8sia3 modulated the sialylation patterns of various substrates.

Fig. 4

a–d St8sia3-KO (−/−) mice showed similar expression levels but larger shifts in the mobility of the major bands for A2AR, AC5, D1R, and D2R in immunoblots of the striatal homogenates compared with WT (+/+). e–h Differences in the mobilities of these striatum-enriched substrates were eliminated after sialidase treatment. The major bands for A2AR, AC5, D2R, and D1R in striatal samples from both genotypes migrated to lower and similar positions after the enzyme treatment (+) compared with untreated groups (−). i–l Intrastriatal injections of an AAV virus expressing mouse ST8SIA3 (AAV-St8sia3) or control (AAV-hrGFP). In the St8sia3-KO striatum, AAV-St8sia3 rescued the obviously decreased sizes of A2AR, AC5, D2R, and D1R to the original size. n = 3 for each genotype (two-tailed unpaired Student’s t-test). All values are presented as the means ± S.E.M. ACTIN was used as an internal loading control