Fig. 5. St8sia3 disruption increased the amount of substrates distributed in lipid rafts and altered the locomotor activities of mice treated with A_2AR and D₂R antagonists.

a–c Lipid raft membrane regions in the striatum of WT (+/+) and St8sia3-KO (−/−) mice were isolated using a nondetergent method and analyzed for substrate distribution by immunoblotting. AC5, A_2AR, D₂R, and D₁R were all dispensed in fraction 3 (raft) along with FLOT1, a well-characterized lipid raft marker. d Quantitative densitometry analysis was used to measure the intensities of substrates in the lipid raft fractions. ST8SIA3 deficiency significantly increased the amounts of AC5, A_2AR, D₂R, and D₁R in lipid rafts. n = 15 for each genotype used for seven independent experiments. **P < 0.01 and ***P < 0.001, compared with WT control (two-tailed unpaired Student’s t-test). All values are presented as the means ± S.E.M. e The A_2AR antagonist SCH 58261 reversed the effects of the D₂R antagonist L-741626 on locomotor activity. L-741626 reduced locomotor activity. The difference between WT and St8sia3-KO mice was observed at a dose of 2.5 mg/kg. SCH 58261 reversed the differences observed between St8sia3-and WT mice at concentrations of 0.1, 1, and 10 mg/kg. n = 8 for each genotype. **P < 0.01, compared with the WT control (two-way ANOVA followed by Bonferroni’s multiple comparison tests). All values are presented as the means ± S.E.M