Fig. 4. ERα inhibits BCa cell invasion by altering circ_0023642/miR-490-5p signaling.

a RNA pull-down assays were performed with biotinylated oligonucleotides designed to target the circ_0023642 circular junction; miR-490-5p was enriched in the pull-down products. b We transfected a miR-490-5p inhibitor into J82 cells and a miR-490-5p mimic into UMUC3 cells. Matrigel invasion assays were performed in J82 and UMUC3 cells transfected with the aforementioned constructs. c An analysis of the TCGA database revealed miR-490-5p expression in BCa tumor tissues compared to normal bladder tissues. d Ectopic miR-490-5p expression reversed the shERα-induced decrease in the invasion of J82 cells. Transfection of UMUC3 cells with a miR-490-5p inhibitor reduced the ERα-induced decrease in cell invasion. Data are presented as means ± SD