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. 2019 Aug 27;9:12418. doi: 10.1038/s41598-019-48889-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of α1 enhances resistance of WT and CP mutant yeast to proteotoxic stress. (a–c) Equal numbers of cells from the indicated yeast strains expressing empty vector (EV) or α1 from a high-copy plasmid were spotted in six-fold serial dilutions on the indicated media and incubated as specified. (d) Cell extracts of WT or pba3Δ yeast expressing empty vector (EV) or α1 from a high-copy plasmid were separated by non-denaturing PAGE before immunoblotting with antibodies against the RP base subunit Rpt1. The positions of doubly-capped CP (RP₂CP), singly-capped CP (RP₁CP), RP, and base subcomplex are shown. Quantification of Rpt1 levels in fully assembled proteasomes (RP₂CP) is shown to the right (n = 10; error bars = s.e.m.). Full-length blot is presented in Supplementary Fig. S8.