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. 2019 Aug 27;9:12421. doi: 10.1038/s41598-019-48741-w

Figure 2.

Figure 2

LPM in SerpinB2−/− and SerpinB2+/+ mice. (a) Peritoneal lavage cells from SerpinB2−/− and SerpinB2+/+ mice were stained with CD11b and F4/80 and analyzed by FACS. Numbers (±SD) show the percentage of SPM and LPM (gates indicated) as a proportion of all peritoneal lavage cells (n = 3 mice per group). The F4/80hi lines indicate the cut-off used to FACS sort LPM. (b) Immunofluorescent antibody staining of FACS-sorted LPM using anti-murine SerpinB2 antibody and FITC-phalloidin (F-actin) imaged using confocal microscopy. All three images for SerpinB2+/+ LPM and SerpinB2−/− LPM are of the same field. (c) Immunoblotting of LPM from SerpinB2+/+ and SerpinB2−/− mice using anti-murine SerpinB2 and anti-GAPDH antibodies. Full length gels are shown in Fig. S15.