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. 2019 Aug 27;9:12421. doi: 10.1038/s41598-019-48741-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RPM migration and length of membrane protrusions. (a) Migration into “scratch wounds” of ex vivo SerpinB2^−/− and SerpinB2^+/+ RPM seeded onto Matrigel-coated wells analyzed using the standard IncuCyte scratch wound assay. Data was generated (for both SerpinB2^−/− and SerpinB2^+/+ RPM) from 3 pools of RPM, each derived from 3 mice, with each pool seeded into 12 wells (i.e. n = 9 SerpinB2^−/− and n = 9 SerpinB2^+/+ mice). The mean of the 3 pools is shown (n = 3). Statistics by repeat measures ANOVA. (b) As for (a) but using ex vivo RPM from SerpinB2^R380A and C57BL/6J mice, one pool of 3 mice (for each strain) and 23 Matrigel coated wells. (c) Western blot of RPM from the indicated mice strains, stained with the murine anti-SerpinB2 antibody. (d) Length of cellular protrusions of RPM within the scratch areas for mice described in (a). The number of cells interrogated is provided within each bar (n), with the longest protrusion from each cell used (i.e. one value for each cell). (e) As for (d) for mice described in (b). (f,g) Representative phase images of the RPM described in (d) at 25 hours post seeding. See also Fig. S3d,e.