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. 2019 Aug 27;9:12419. doi: 10.1038/s41598-019-48838-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Live-cell Stimulated Emission Depletion (STED) nanoscopy of mitochondrial cristae in HeLa cells. HeLa cells stably expressing COX8A-SNAP fusion proteins were labeled using SNAP-Cell SiR and visualized with STED nanoscopy. (a) Overview of HeLa cells. Shown is a comparison between confocal and STED resolution. (b) Left: Magnifications of the areas indicated in the overview image. Right: Fluorescence intensity line profiles measured as indicated in the magnifications. STED images show unprocessed raw data without background subtraction. Scale bars: 1 µm.