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. 2019 Aug 27;9:12406. doi: 10.1038/s41598-019-48883-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fast kinetics of rPrP aggregation at room temperature followed by LS in a stopped-flow instrument. (A) Time course and (B) rates of aggregation of rPrP (2 μM) alone or with DHFR mRNA at various rPrP to RNA ratios. (C) Time course and (D) rates of rPrP (2 μM) aggregation after seeding the reaction with 10% (v/v) SN from rPrP aggregation reactions with various rPrP to DHFR mRNA ratios. The inset in (C) shows LS curves beyond 150 sec. AU – arbitrary units. The error bars in (B,D) indicate standard deviation from three independent measurements.