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Distribution of rPrP in the supernatant (SN) and the pellet (P) fractions in the presence of RNA, assessed by SDS-PAGE. rPrP samples (10 μM) with and without RNAs at various ratios were incubated 10 min at room temperature and then centrifuged at 20,000 g. Equal volumes of supernatant and buffer equilibrated pellet were ran on a 15% SDS acrylamide gel (A). The amount of protein in each sample was estimated as a percentage of total protein available in each supernatant/pellet pair (B).
