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. 2019 Aug 27;9:12406. doi: 10.1038/s41598-019-48883-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CD spectra of rPrP (5 μM) aggregated in the presence of different RNAs (A) domain V rRNA, (B) DHFR mRNA, (C) GFP+ mRNA, at 1:0.05 protein to RNA ratio. For each sample, three spectra were collected; protein alone (red trace); RNA alone (green); rPrP-RNA complex (blue trace). The sum of the protein alone and RNA alone spectra produced the cumulative spectra (yellow trace); RNA spectra subtracted from the complex spectra (blue trace) generated the difference spectra (dotted line).