Table 1.

Summary of the effects of cyclin A2 degron mutations to its ubiquitination

Degron mutation	APC/CCdc20	APC/CMCC	Degrons present
KEN box mutations
ΔK alone	Same as WT	Lower	D1D2A
ΔKD1	Same as WT	Lower	D2A
ΔKD2	Lower	Lower	D1A
ΔKA	Same as WT	No activity	D1D2
ΔKD1D2	No activity	No activity	A
ΔKD1A	Same as WT	No activity	D2
ΔKD2A	No activity	No activity	D1
ΔKD1D2A	No activity	No activity	None
D1 box mutations
ΔD1 alone	Higher	Same as WT	KD2A
ΔKD1	Same as WT	Lower	D2A
ΔD1D2	No activity	No activity	KA
ΔD1A	Higher	Lower	KD2
ΔKD1D2	No activity	No activity	A
ΔKD1A	Same as WT	No activity	D2
ΔKD2A	No activity	No activity	K
ΔKD1D2A	No activity	No activity	None
D2 box mutations
ΔD2 alone	Lower	Lower	KD1A
ΔKD2	Lower	Lower	D1A
ΔD1D2	No activity	No activity	KA
ΔD2A	Lower	No activity	KD1
ΔKD1D2	No activity	No activity	A
ΔKD2A	No activity	No activity	D1
ΔD1D2A	No activity	No activity	K
ΔKD1D2A	No activity	No activity	None
ABBA motif mutations
ΔA alone	Higher	Lower	KD1D2
ΔKA	Same as WT	No activity	D1D2
ΔD1A	Higher	Lower	KD2
ΔD2A	Lower	No activity	KD1
ΔKD1A	Same as WT	No activity	D2
ΔKD2A	No activity	No activity	D1
ΔD1D2A	No activity	No activity	K
ΔKD1D2A	No activity	No activity	None

Any combinations with the D2 box mutated resulted in a reduction or elimination of cyclin A2 ubiquitination by both APC/C^Cdc20 and APC/C^MCC