FIGURE 1.

Experimental plan and effects of 7-days BzATP administration on whole oxygen consumption. Adult (15 weeks-old) female C57BL/6J mice were daily i.p., administered with PBS or 1 mg/Kg BzATP for 7 days. At day 5, vehicle (n = 5) and BzATP-treated mice (n = 5), were introduced in the metabolic chambers to adapt for 24 h, and then subjected to IC recording for 48 h (days 6 and 7). (A) Schematic representation of experimental plan and execution. (B) Mean (± SEM) body weight (g) collected during the IC analysis for 48 h. (C) Mean (± SEM) of 48 h metabolic activity expressed as volume of oxygen consumption rate (VO₂) (ml/h/Kg), during the entire light/dark cycle. Black line below the recording time (X-axis) indicates the dark phase.