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. 2019 Aug 13;6(4):ENEURO.0058-19.2019. doi: 10.1523/ENEURO.0058-19.2019

Figure 4.

Figure 4.

Electrophysiological properties of AOB EGCs. A, EGC morphologic reconstructions. B1–D1, Responses of three representative EGCs to current clamp challenges. Scale bars: 10 mV, 500 ms. B2–D2, Responses of the same 3 EGCs to a series of command potential steps from –70 mV in voltage clamp. Hyperpolarizing responses were also recorded, but are not shown here. Scale bars: 1 nA, 100 ms. E, Spike rate input-output curves for EGCs. Dashed purple line indicates the mean ± SE for EGCs, and dashed green for IGCs (from Fig. 2E; N for EGCs = 33, two-way ANOVA compared to IGCs: p = 1.48 × 10−5 main effect of cell type, p = 0.0051 interaction between cell type and stimulus intensity). F, Spike accommodation index for EGCs (N = 33, two-way ANOVA compared to IGCs: p = 0.83 main effect of cell type. p = 0.113 interaction between cell type and stimulus intensity). G, Maximal IH sag potential (N = 33, compared to IGCs p = 1.48 × 10−9 by Wilcoxon rank-sum test). H, Input-output curves for peak inward voltage-gated Na+ currents (N = 29, two-way ANOVA compared to IGCs: p = 0.518 main effect of cell type, p = 0.0267 interaction between cell type and stimulus intensity). I, Input-output curves for the ratio of peak voltage-gated Na+ to peak voltage-gated K+ currents (N = 29, two-way ANOVA compared to IGCs: p = 0.0048 main effect of cell type, p = 0.0916 interaction between cell type and stimulus intensity). J, Input-output curve for normalized voltage-gated K+ current inactivation (N = 29, two-way ANOVA compared to IGCs: p = 0.0381 main effect of cell type, p = 0.1823 interaction between cell type and stimulus intensity). K, Representative recordings of spontaneous synaptic currents (command potential –70 mV) before and during application of gabazine, AP5, NBQX, and MCPG (N = 5). L, Blockade of net spontaneous synaptic currents during pharmacological blockade (holding potential –70 mV). Asterisks indicate p < 0.05 by multiple comparisons of mean ranks, one-way ANOVA. M, sEPSC frequency (N = 29, p = 2.84 × 10−6 Wilcoxon rank-sum test). Purple bars refer to EGCs, green bars to IGCs. N, sEPSC amplitude (N = 29, p = 9.53 × 10−13 Wilcoxon rank-sum test).