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. 2019 Aug 26;6(4):ENEURO.0448-18.2019. doi: 10.1523/ENEURO.0448-18.2019

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Copyright © 2019 Kaiser and Feng

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 6. — Blood monocyte profiling in Tmem119 knock-in mice. A, Experimental approach for flow cytometry of monocytes isolated from whole blood. B, C, Representative flow cytometry analysis of EGFP expression in pre-gated single live CD45⁺ cells in WT control (B) and Tmem119-EGFP^+/− mice (C). One of three experiments is shown (N = 3 mice). Numbers in or adjacent to outlined areas indicate percentage cells in each gate. D, Adult mice of Tmem119-CreERT2^+/−; Ai14(RCL-tdT)-D^+/− mice received tamoxifen (TAM) per os (p.o.) for 3 d. The mice were sacrificed 7 d after the last dose for flow cytometry (FC). E, F, Representative flow cytometry analysis of tdTomato expression in pre-gated single live CD45⁺ cells in Ai14(RCL-tdT)-D^+/− (E) and Tmem119-CreERT2^+/−; Ai14(RCL-tdT)-D^+/− mice (F). One of two independent experiments is shown (N = 3 mice).