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. 2019 Aug 27;9:12442. doi: 10.1038/s41598-019-48866-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Localization of G309C, I580V, G645R, D742Y and the enzymatically inactive D350N in the pmc1Δ pmr1Δ cnb1Δ strain PAP4920 is rescued by co-expression of wt hSPCA1a. Live cell bioimaging of yeast strain PAP4920 co-expressing BFP tagged wt hSPCA1a and GFP tagged hSPCA1a variants or the endogenous Pmc1 from the replicative vector pPAP4997 at 30 °C. Cells were grown as described in Fig. 9. All images were taken at 1000x magnification. Left column shows differential interference contrast (DIC). The middle column (GFP) shows GFP fluorescence in the same cells. The third column (SPCA1a-BFP) shows BFP fluorescence in the same cells. The single images shown represent one out of at least five.