Figure 5.

Complementation of the manganese sensitive phenotype of the pmr1Δ pmc1Δ cnb1Δ yeast host strain by replicative hSPCA1a mutants. Yeast strain PAP4920 expressing no hSPCA1a (Empty Vector, EV), wt hSPCA1a (WT) or the indicated HHD hSPCA1a variants from the integrative vector (pPAP5480, Supplementary Fig. S1) were grown at 30 °C in 96-well plates in liquid media with galactose as carbon source supplemented with 20 mM CaCl₂, and MnSO₄ ranging from 0 to 2.0 mM in increments of 0.1 mM as indicated. Each culture was grown at 30 °C, in triplicates, and average OD₄₅₀ was plotted with respect to time of inoculation.