. 2019 Aug 27;9:12442. doi: 10.1038/s41598-019-48866-y

Table 1.

Phenotypes of homozygous ATP2C1 alleles.

	BAPTA resistance^a (liquid) (solid)				Mn²⁺ resistance^a (liquid) (solid)				Ca²⁺ sensitivity^b (liquid) (solid)				Temperature sensitivity^c		Protein accumulation^d	Protein localization	UPR^e
Copy Number ^f	LC		HC		LC		HC		LC		HC		LC	HC	HC	HC
EV	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	−	Low
WT	++	++	++	++	++	++	++	++	0	0	0	0	HS	HS	High	Golgi	Low
A304T	0	0	++	+	0	0	0	0	0	0	+	0	0	0	High	Golgi	High
G309C	0	0	+	+	0	0	0	0	0	0	+	+	CS	CS	High	Vacuole	Low
L318P	++	++	++	++	0	0	0	0	+	+	+	+	CS	CS	High	Golgi	High
C344Y	++	++	++	++	0	0	+	+	+	0	0	0	CS	CS	Low	Golgi	High
C411R	0	0	+	+	0	0	0	0	0	0	0	+	CS	CS	Low	Golgi	High
C490F	0	0	++	+	0	0	0	0	0	0	+	+	0	HS	Low	Golgi	High
T570I	+	0	+	+	0	0	0	0	+	0	0	+	HS	0	Low	Golgi	High
I580V	++	++	++	++	++	++	++	++	0	0	0	0	HS	HS	High	Vacuole	High
L584P	+	+	++	++	0	0	0	0	0	0	+	0	CS	CS	Low	Golgi	High
M641R	0	0	+	+	0	0	0	0	0	0	+	+	CS	CS	Low	Golgi	High
G645R	0	0	+	+	0	0	0	0	0	0	+	+	0	CS	High	Vacuole	High
T709M	+	0	+	+	0	0	0	0	0	0	+	+	CS	CS	High	Golgi	Low
D742Y	0	0	+	+	0	0	0	0	0	0	0	+	CS	CS	High	Vacuole	High
G789R	0	0	0	0	0	0	0	0	0	0	0	0	0	0	Low	Golgi	High
Pmr1	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	Low	Golgi	Low
Pmc1	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	High	Vacuole	Low

^aBAPTA resistance and Mn²⁺ resistance were based on data in Figs 2–6 (30 °C only) and given a value of 0 if complementation was empty vector like, “+” if growth was intermediary and “++” if growth was wt-like. ^bCa²⁺ sensitivity was determined from Figs 2, 3 and 6 (30 °C only) and given a value of 0 if no Ca²⁺ dependent inhibition was observed or a “+” if growth was inhibited at media containing 20 mM CaCl₂ or 0 mM BAPTA.^cTemperature sensitivity was categorized from the spot assay in Fig. 6, as being either Cold Sensitive (CS), Heat Sensitive (HS) or temperature independent (0). ^dProtein accumulation was based on Fig. 8 and given two values: High if protein accumulation was wt-like, Low if protein accumulation was lower than wt. ^eUPR data from Fig. 14 were given two values: High if the induced UPR level was at least two times greater than that of wt and Low if the induced UPR level was less than two times that of wt. ^eCopy Number indicates the expression from the integrative (Low Copy, LC) or from the replicative (High Copy, HC) plasmids. Liquid, cells were grown in liquid medium in microplates; solid, cells were spotted on solid agar medium; ND, not determined.