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. 2019 Aug 16;12(11):1468–1479. doi: 10.1016/j.tranon.2019.07.019

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Phenotypic characterization of tumor-infiltrating monocytes and macrophages in HCC and CCA patients. (A) Bivariate dot plot histograms illustrating the phenotypic characterization of monocytes (blue events) and macrophages (orange events) infiltrating tumor biopsies. (B) Comparison among HCC and CCA tumor-infiltrating macrophages and monocytes expression levels of CD274 (PD-L1), CD200R, CD206, CD80, and CD163 markers, measured as MFI. The results are given by median with interquartile range. Statistically significant differences were considered when P < .05; ∗between the groups indicated in the figure. (C) Boxplots with the mRNA expression levels of IL-10 and TNFα by tumor-infiltrating macrophages (purified by cell sorting) in HCC and CCA. The results are given as median with interquartile range.