FIG 2.
The myo5Δ mutant accumulates high levels of amino acids. Log-phase cells of the wild type, myo5Δ mutant, and the myo5Δ complemented strain (MYO5) were incubated with the indicated 3H-labeled amino acids, and the time course of intracellular accumulation of each amino acid was determined. The experiments were repeated three times, and the error bars represent the standard deviations. Two-way ANOVA with Dunnett’s multiple-comparison test was used to compare the differences at the indicated time points between the corresponding R265 strain and myo5Δ mutant (*, P < 0.0332; **, P < 0.0021; ***, P < 0.0002; ****, P < 0.0001).